DSMZ 1028 . THERMINCOLA MEDIUM (CO)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter Gassing station Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 0.979 18.311
MgCl2 x 6 H2O 0.33 g 0.323 1.59
CaCl2 x 2 H2O 0.10 g 0.098 0.666
KCl 0.33 g 0.323 4.336
KH2PO4 0.50 g 0.49 3.599
Wolfe's mineral elixir 1.00 ml - -
Na-acetate 0.20 g 0.196 2.388
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
NaHCO3 0.50 g 0.49 5.829
Na2CO3 0.50 g 0.49 4.62
Na2S x 9 H2O 1.00 g 0.979 4.078
Wolin's vitamin solution 20.00 ml - -
Yeast extract 0.20 g 0.196 -
Distilled water 1000.00 ml - -
1 Dissolve ingredients except carbonates, sulfide, vitamins and yeast extract, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonates and sulfide to the medium, dispense under 100% carbon monoxide gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 10 ml medium in 50 ml bottles) and autoclave. Prior to inoculation add vitamins (sterilized by filtration) and yeast extract from sterile anoxic stock solutions prepared under 100% N2 gas. If the medium is not completely reduced after autoclaving (remains pink) add 0.30 g/l sodium sulfide from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 8.0 with a sterile anoxic solution of 1 N HCl.
Solution occurs in 23 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 12.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes, Serum bottle
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Gas composition

  • 90 % CO
  • 10 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NH4Cl 0.979 18.311
Na2S x 9 H2O 0.979 4.078
NaHCO3 0.49 5.829
Na2CO3 0.49 4.62
KH2PO4 0.49 3.599
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain