DSMZ 1029 . BBL ACTINOMYCES BROTH

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Wheaton tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
BBL Actinomyces broth (BBL 210920) 57 g 57 -
Distilled water 1000 ml - -
1 final pH 6.9
2 Distribute into screw-capped Wheaton tubes. For anaerobic handling prepare and distribute into Hungate tubes by using the Hungate technique.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Potassium Phosphate 15.00 g 15 70.666
Ammonium Sulfate 1.00 g 1 -
Magnesium sulfate 0.20 g 0.2 1.662
Calcium Chloride 0.01 g 0.01 0.068
Infusion Broth 25.00 g 25 -
Dextrose 5.00 g 5 25.231
L-Cysteine HCl 1.00 g 1 -
Pancreatic digest of casein 4.00 g 4 -
Yeast extract 5.00 g 5 -
Starch
(soluble)
1.00 g 1 -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Wheaton tubes
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Associated strains

All strains for this medium: 7
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Infusion Broth 1.425 -
Potassium Phosphate 0.855 4.028
Dextrose 0.285 1.438
Yeast extract 0.285 -
Pancreatic digest of casein 0.228 -
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Cultivation metadata from 7 strains BacDive

pH 6 - 8 3 strains
pH 10 - 12 1 strain
phyla 1 7 strains
oxygen facultative anaerobe 5 strains
oxygen microaerophile 1 strain
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