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DSMZ

DSMZ 1033 . THERMUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1033 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(C6H9NO6) 		0.10 g 0.1 0.523
CaSO4 x 2 H2O 0.06 g 0.06 0.348
MgSO4 x 7 H2O 0.10 g 0.1 0.406
NaCl 8.00 mg 0.008 0.137
KNO3 0.10 g 0.1 0.989
NaNO3 0.69 g 0.69 8.118
Na2HPO4 0.10 g 0.1 0.704
Traceelements Solution 10.00 ml - -
FeCl3 x 6 H2O
(0.17 mM) 		10.00 ml 4.6e-4 0.002
Tryptone
(Bacto Trypton 211705) 		1.00 g 1 14.069
Yeast extract
(Oxoid LP0021, BD 212750) 		1.00 g 1 -
Agar 15.00 g 15 44.599
Distilled water 1000.00 ml - -
1 Dissolve the nitrilotriacetic acid (C6H9NO6), adjust the pH to 8.0 with 1 M NaOH.
2 Dissolve the remaining ingredients in the nitrilotriacetic acid solution and adjust to pH 8.2 with 1 M NaOH.
3 Autoclave at 121° C for 15 minutes.

Traceelements Solution #

1000
Solution occurs in 1 other media (first defined in Medium 1033).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnSO4 x H2O 0.22 g 0.22 1.302
ZnSO4 x 7 H2O 0.05 g 0.05 0.174
H3BO3 0.05 g 0.05 0.809
CuSO4 x 5 H2O 2.50 mg 0.003 0.01
Na2MoO4 x 2 H2O 2.50 mg 0.003 0.01
CoCl2 x 6 H2O 4.60 mg 0.005 0.035
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 6

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Tryptone 1 14.069
Yeast extract 1 -
NaNO3 0.69 8.118
KNO3 0.1 0.989
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Cultivation metadata from 6 strains BacDive

phyla 2 6 strains
oxygen aerobe 2 strains
oxygen obligate aerobe 2 strains
temperature thermophilic 6 strains