Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
NH4Cl | 0.5 | g | ||
|
KH2PO4 | 0.2 | g | ||
|
MgCl2 x 6 H2O | 0.1 | g | ||
|
KCl | 0.2 | g | ||
|
Trace element solution SL-10 | 1.0 | ml | ||
|
Selenite-tungstate solution | 1.0 | ml | ||
|
Na2CO3 | 1.0 | g | ||
|
NaHCO3 | 7.6 | g | ||
|
NaCl | 10.0 | g | ||
|
Yeast extract | 0.2 | g | ||
|
Cellobiose | 3.0 | g | ||
|
Na2S x 9 H2O | 0.5 | g | ||
|
Distilled water | 1000.0 | ml | ||
1 Dissolve ingredients except cellobiose and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose after autoclaving from an anoxic stock solution prepared under 100% N2 gas sterilized by filtration and sulfide from a sterile anoxic stock solution autoclaved under 100% N2 gas. Adjust pH of the complete medium to pH 8.8-9.0. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | ||
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
ZnCl2 | 70.0 | mg | ||
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
H3BO3 | 6.0 | mg | ||
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
Distilled water | 990.0 | ml | ||
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Compound | Amount | Unit | |
---|---|---|---|
|
NaOH | 0.5 | g |
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
Na2WO4 x 2 H2O | 4.0 | mg |
|
Distilled water | 1000.0 | ml |