Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Sea Salt
(SIGMA) |
30.0 | g | 29.851 | - | |
NaNO3 | 1.0 | g | 0.995 | 11.707 | |
Wolfe's mineral elixir | 2.0 | ml | - | - | |
VOSO4 x 5 H2O
(0.01% w/v) |
0.5 | ml | 5.0e-5 | 2.0e-4 | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
FeSO4 x 7 H2O | 0.1 | g | 0.1 | 0.358 | |
NaHCO3 | 1.0 | g | 0.995 | 11.845 | |
Yeast extract | 0.5 | g | 0.498 | - | |
Na-pyruvate | 1.0 | g | 0.995 | 9.042 | |
Wolin's vitamin solution (10x) | 2.0 | ml | - | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve ingredients (except ferrous sulfate, bicarbonate, yeast extract, pyruvate and vitamins), adjust pH to 7.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense the medium under same gas atmosphere into Hungate-type tubes or serum vials and autoclave. Add ferrous sulfate, bicarbonate, yeast extract, pyruvate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Prepare the stock solution of ferrous sulfate by dissolving 1% (w/v) FeSO4 x 7 H2O in 0.1 N H2SO4. The stock solutions of pyruvate and vitamins should be sterilized by filtration. Adjust pH of the complete medium to 6.8-7.0. | |||||
2 Note: It may be necessary to add 10-20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under 100% N2 and filter-sterilized), if the medium is not completely reduced after inoculation. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgSO4 x 7 H2O | 30.00 | g | 30 | 121.716 | |
MnSO4 x H2O | 5.00 | g | 5 | 29.583 | |
NaCl | 10.00 | g | 10 | 171.116 | |
FeSO4 x 7 H2O | 1.00 | g | 1 | 3.597 | |
CoCl2 x 6 H2O | 1.80 | g | 1.8 | 13.863 | |
CaCl2 x 2 H2O | 1.00 | g | 1 | 6.802 | |
ZnSO4 x 7 H2O | 1.80 | g | 1.8 | 6.26 | |
CuSO4 x 5 H2O | 0.10 | g | 0.1 | 0.401 | |
AlK(SO4)2 x 12 H2O | 0.18 | g | 0.18 | 0.379 | |
H3BO3 | 0.10 | g | 0.1 | 1.617 | |
Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
(NH4)2Ni(SO4)2 x 6 H2O | 2.80 | g | 2.8 | 7.089 | |
Na2WO4 x 2 H2O | 0.10 | g | 0.1 | 0.303 | |
Na2SeO4 | 0.10 | g | 0.1 | 0.529 | |
Distilled water | 1000.00 | ml | - | - | |
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 16.09.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.8 - 7.0 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Sea Salt | 29.851 | - |
NaHCO3 | 0.995 | 11.845 |
NaNO3 | 0.995 | 11.707 |
Na-pyruvate | 0.995 | 9.042 |
Yeast extract | 0.498 | - |
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