DSMZ 1042 . VULCANIBACILLUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA)
30.0 g 29.326 -
NaNO3 1.0 g 0.978 11.501
Wolfe's mineral elixir 2.0 ml - -
VOSO4 x 5 H2O
(0.01% w/v)
0.5 ml 4.9e-5 1.9e-4
Sodium resazurin
(0.1% w/v)
0.5 ml 4.9e-4 0.002
FeSO4 x 7 H2O 0.1 g 0.098 0.352
NaHCO3 1.0 g 0.978 11.636
Yeast extract 0.5 g 0.489 -
Na-pyruvate 1.0 g 0.978 8.883
Wolin's vitamin solution 20.0 ml - -
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except ferrous sulfate, bicarbonate, yeast extract, pyruvate and vitamins), adjust pH to 7.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense the medium under same gas atmosphere into Hungate-type tubes or serum vials and autoclave. Add ferrous sulfate, bicarbonate, yeast extract, pyruvate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Prepare the stock solution of ferrous sulfate by dissolving 1% (w/v) FeSO4 x 7 H2O in 0.1 N H2SO4. The stock solutions of pyruvate and vitamins should be sterilized by filtration. Adjust pH of the complete medium to 6.8-7.0.
2 Note: It may be necessary to add 10-20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under 100% N2 and filter-sterilized), if the medium is not completely reduced after inoculation.
Solution occurs in 23 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 12.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sea Salt 29.326 -
NaHCO3 0.978 11.636
NaNO3 0.978 11.501
Na-pyruvate 0.978 8.883
Yeast extract 0.489 -
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 3 strains
nutrition chemoorganoheterotroph 1 strain
oxygen anaerobe 3 strains
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