DSMZ 1043 . IGNISPHAERA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Serum bottle Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.286 9.731
CaCl2 x 2 H2O 74.00 mg 0.073 0.498
MgSO4 x 7 H2O 0.28 g 0.277 1.124
KH2PO4 0.28 g 0.277 2.035
Yeast extract
(OXOID)
0.10 g 0.099 -
Trypticase peptone
(BD BBL)
2.00 g 1.978 -
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
FeCl3 x 6 H2O
(0.1% w/v in 0.2 N HCl)
0.50 ml 4.9e-4 0.002
L-Cysteine HCl x H2O 0.30 g 0.297 1.689
Starch
(soluble)
2.00 g 1.978 -
Na2S x 9 H2O 0.30 g 0.297 1.235
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except ferric chloride, cysteine, starch and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add ferric chloride and solid cysteine, then adjust pH to 6.3. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 20 ml medium in 50 ml bottles) and autoclave. Prior to inoculation add starch and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 6.5, if necessary.
Solution occurs in 170 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 12.04.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Gassing station, Serum bottle
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Starch 1.978 -
Trypticase peptone 1.978 -
(NH4)2SO4 1.286 9.731
L-Cysteine HCl x H2O 0.297 1.689
Na2S x 9 H2O 0.297 1.235
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
nutrition heterotroph 1 strain
oxygen anaerobe 1 strain
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