DSMZ 1047 . DIFCO RAKA-RAY NO.3 MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Fructobacillus tropaeoli DSM 23246

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 5.00 g 5 -
Tryptone 20.00 g 20 281.373
Liver Concentrate 1.00 g 1 -
Maltose 10.00 g 10 29.214
Fructose 5.00 g 5 27.753
Glucose 5.00 g 5 27.753
Betaine Hydrochloride 2.00 g 2 -
Diammonium Citrate 2.00 g 2 8.842
Potassium Aspartate 2.50 g 2.5 -
Magnesium sulfate 0.98 g 0.98 8.142
Manganese Sulfate 0.42 g 0.42 2.485
Dipotassium Phosphate 2.00 g 2 11.483
N-Acetylglucosamine 0.50 g 0.5 2.26
Potassium Glutamate 2.50 g 2.5 -
Agar 16.00 g 16 47.572
Distilled water 1000.00 ml - -

Metadata

Last modified: 23.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.4
Description:

For isolation of lactic acid bacteria encountered in beer and the brewing process

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Associated strains

All strains for this medium: 3
Fructobacillus tropaeoli DSM 23246

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 20 281.373
Agar 16 47.572
Maltose 10 29.214
Glucose 5 27.753
Fructose 5 27.753
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Cultivation metadata from 3 strains BacDive

pH 2 - 4 1 strain
pH 4 - 6 1 strain
phyla 1 3 strains
oxygen facultative anaerobe 2 strains
oxygen microaerophile 2 strains
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