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DSMZ

DSMZ 1049 . SELENATE REDUCER MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1049 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 100.0 ml - -
Solution B 1.0 ml - -
Solution C 2.5 ml - -
Solution D 1.0 ml - -
Solution E 0.1 ml - -
Solution F 0.5 ml - -
Solution G 1.0 ml - -
Solution H 1.0 ml - -
1 After cooling of medium A, add per 100 ml: 1.0 ml solution B, 2.5 ml solution C, 1.0 ml solution D, 0.1 ml solution E, 0.5 ml solution F, 1.0 ml solution G and 1.0 ml solution H.
2 Adjust pH to 7.0.
3 It is recommended to cultivate strain DSM 17993 on agar plates which may be prepared under aerobic conditions. Plates are then preincubated under anaerobic conditions prior to inoculation. They will stay pink coloured.
4 For liquid medium, prepare solution A, distribute to anaerobic cultivation vessels, heat to the boil and cool to room temperature while gassing with N2 : CO2 (80:20). Close vessels and autoclave. Prepare solutions B, C, D, E and H under anaerobic conditions and autoclave. Solutions F and H are filter sterilized and flushed with N2. Selenate (solution B) might be replaced by nitrate (solution: 1.00 g KNO3 in 10 ml).

Solution A #

940
Compound Amount Unit Conc. [g/L] Conc. [mM]
DL-Na-lactate 1.12 g 1.191 10.633
KCl 1.30 g 1.383 18.551
KH2PO4 0.20 g 0.213 1.563
NaCl 23.00 g 24.468 418.687
NH4Cl 0.50 g 0.532 9.944
CaCl2 x 2 H2O 0.10 g 0.106 0.724
MgCl2 x 6 H2O 3.00 g 3.191 15.698
Resazurin 0.50 mg 5.3e-4 0.002
Agar 15.00 g 15.957 47.446
Distilled water 940.00 ml - -

Solution B #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO4 1.89 g 189 1000.26
Distilled water 10.00 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1 g 100 1190.38
Distilled water 10 ml - -

Solution D #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.1 g 10 41.635
Distilled water 10.0 ml - -

Solution E #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 3 mg 0.03 0.173
Na2WO4 8 mg 0.08 0.272
Distilled water 100 ml - -

Solution F #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin solution 1 ml - -

Solution G #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SL-10 1 ml - -
Distilled water 9 ml - -

Solution H #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
1,4-Naphthoquinone 2.0 mg 0.02 0.126
Hemin 0.5 mg 0.005 0.008
Distilled water 100.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Vitamin solution #

1000
Solution occurs in 15 other media (first defined in Medium 461).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Filter
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 22.867 391.296
Agar 14.914 44.342
MgCl2 x 6 H2O 2.983 14.671
NaHCO3 2.336 27.813
Na2SeO4 1.766 9.348
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain