1049 .
SELENATE REDUCER MEDIUM
What is this page?
Equipment needed:
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Solution A | 100.0 | ml | ||
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Solution B | 1.0 | ml | ||
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Solution C | 2.5 | ml | ||
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Solution D | 1.0 | ml | ||
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Solution E | 0.1 | ml | ||
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Solution F | 0.5 | ml | ||
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Solution G | 1.0 | ml | ||
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Solution H | 1.0 | ml | ||
| 1 After cooling of medium A, add per 100 ml: 1.0 ml solution B, 2.5 ml solution C, 1.0 ml solution D, 0.1 ml solution E, 0.5 ml solution F, 1.0 ml solution G and 1.0 ml solution H. | |||||
| 2 Adjust pH to 7.0. | |||||
| 3 It is recommended to cultivate strain DSM 17993 on agar plates which may be prepared under aerobic conditions. Plates are then preincubated under anaerobic conditions prior to inoculation. They will stay pink coloured. | |||||
| 4 For liquid medium, prepare solution A, distribute to anaerobic cultivation vessels, heat to the boil and cool to room temperature while gassing with N2 : CO2 (80:20). Close vessels and autoclave. Prepare solutions B, C, D, E and H under anaerobic conditions and autoclave. Solutions F and H are filter sterilized and flushed with N2. Selenate (solution B) might be replaced by nitrate (solution: 1.00 g KNO3 in 10 ml). | |||||
Solution A #
940
| Compound | Amount | Unit | |
|---|---|---|---|
|
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DL-Na-lactate | 1.12 | g |
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KCl | 1.30 | g |
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KH2PO4 | 0.20 | g |
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NaCl | 23.00 | g |
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NH4Cl | 0.50 | g |
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CaCl2 x 2 H2O | 0.10 | g |
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MgCl2 x 6 H2O | 3.00 | g |
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Resazurin | 0.50 | mg |
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Agar | 15.00 | g |
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Distilled water | 940.00 | ml |
Solution H #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
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1,4-Naphthoquinone | 2.0 | mg |
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Hemin | 0.5 | mg |
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Distilled water | 100.0 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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HCl
(25%) |
10.0 | ml | ||
|
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FeCl2 x 4 H2O | 1.5 | g | ||
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ZnCl2 | 70.0 | mg | ||
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MnCl2 x 4 H2O | 100.0 | mg | ||
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H3BO3 | 6.0 | mg | ||
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CoCl2 x 6 H2O | 190.0 | mg | ||
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CuCl2 x 2 H2O | 2.0 | mg | ||
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NiCl2 x 6 H2O | 24.0 | mg | ||
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Na2MoO4 x 2 H2O | 36.0 | mg | ||
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Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Vitamin B12 | 50 | mg | ||
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Pantothenic acid | 50 | mg | ||
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Riboflavin | 50 | mg | ||
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Pyridoxamine hydrochloride | 10 | mg | ||
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Biotin | 20 | mg | ||
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Folic acid | 20 | mg | ||
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Nicotinic acid | 25 | mg | ||
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Nicotine amide | 25 | mg | ||
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alpha-lipoic acid | 50 | mg | ||
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p-Aminobenzoic acid | 50 | mg | ||
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Thiamine-HCl x 2 H2O | 50 | mg | ||
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Distilled water | 1000 | ml | ||
| 1 Stir for some hours, filter sterilize the solution. | |||||