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104. PYG MEDIUM (MODIFIED)

Culture medium recipe

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* Modified for Actinomyces massiliensis DSM 23047
beta

Add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, and 0.1% soluble starch; anaerobic

Solution occurs in 1 other media (first defined in medium 104 )
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.0 g 4.995 -
2 5.0 g 4.995 -
3 10.0 g 9.99 -
4 5.0 g 4.995 -
5 5.0 g 4.995 27.726
6 2.0 g 1.998 11.471
7 1.0 ml - -
8 Salt solution 40.0 ml - -
9 1.0 mg 1.0e-3 0.004
10 Vitamin K1 solution 0.2 ml - -
11 Haemin solution 10.0 ml - -
12 0.5 g 0.5 2.844
13 950.0 ml 947.349 52585.8
14 , required for pH adjustment
(8 N)
x - -
17
(optional)
0.2 g/l 0.2 1.052
* 0.4 % - -
Instructions:
  1. The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave.
Solution occurs in 6 other media (first defined in medium 104 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 0.25 1.701
2 0.50 g 0.5 2.029
3 1.00 g 1 5.741
4 1.00 g 1 7.348
5 10.00 g 10 119.038
7 1000.00 ml 998.207 55408.8
Solution occurs in 2 other media (first defined in medium 104 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 50 mg 0.495 -
2
(1 N)
1 ml 21.089 527.266
3 100 ml 988.324 54860.2
Instructions:
  1. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store refrigerated.
Solution occurs in 2 other media (first defined in medium 104 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 0.1 ml - -
3
(95 %)
20.0 ml 750.5 16291
Instructions:
  1. Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 416
Strains with modifications: 394
Actinomyces massiliensis DSM 23047

  0.4 % glucose

Light

Intensity 0.4 % glucose lux

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.111 55348
Yeast extract 9.99 -
Glucose 8.995 49.929
Trypticase peptone 4.995 -
Peptone 4.995 -
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Cultivation metadata from 416 strains BacDive

halophily not 12 strains
halophily slightly 7 strains
halophily moderate 5 strains
pH 6 - 8 33 strains
pH 4 - 6 22 strains
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