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104b. PY + X MEDIUM

Culture medium recipe

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* Modified for Alkaliphilus oremlandii DSM 21761
beta

Replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 - 8.0.

Equipment needed: Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(BD BBL)
5.0 g 5 -
2
(pepsin-digested)
5.0 g 5 -
3 10.0 g 10 -
4 Salt solution 40.0 ml - -
5
(0.1% w/v)
0.5 ml 5.0e-4 0.002
6 0.5 g 0.5 2.847
8 1000.0 ml 998.207 55408.8
13 , required for pH adjustment
(5% w/v)
x - -
* 5.0 g/l - -
Instructions:
  1. Dissolve ingredients (except cysteine and glucose) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH to 7.0, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose or any other substrate from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.8 - 7.0, if necessary.
Solution occurs in 6 other media (first defined in medium 104 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 0.25 1.701
2 0.50 g 0.5 2.029
3 1.00 g 1 5.741
4 1.00 g 1 7.348
5 10.00 g 10 119.038
7 1000.00 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 7.5 - 8.0
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 121
Strains with modifications: 119
Alkaliphilus oremlandii DSM 21761

  5.0 g/l D-fructose
  D-Glucose
  7.5 - 8.0

Gas composition

  • 100 % N2

Light

Intensity 5.0 g/l D-fructose lux

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Distilled water 1038.14 57625.2
Yeast extract 10 -
D-Fructose 5 27.754
Trypticase peptone 5 -
Peptone from meat 5 -
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Cultivation metadata from 121 strains BacDive

halophily not 11 strains
halophily slightly 6 strains
halophily moderate 3 strains
halophily extreme 1 strain
pH 6 - 8 27 strains
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Similar media

104a. ANAEROBACULUM MEDIUM 69 %
104c. PY + X MEDIUM (N2/CO2) 66 %
712. PSEUDOBUTYRIVIBRIO MEDIUM 63 %
152. NICOTINIC ACID MEDIUM 60 %
104d. MALEDIVIBACTER MEDIUM (BETAINE) 58 %

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