104c .
PY + X MEDIUM (N2/CO2)
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Selected strain: Dysosmobacter welbionis DSM 106889
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
37 °C
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase peptone
(BD BBL) |
5.0 | g | ||
|
|
Meat peptone
(pepsin-digested) |
5.0 | g | ||
|
|
Yeast extract | 10.0 | g | ||
|
|
Salt solution | 40.0 | ml | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
|
Na2CO3 | 1.0 | g | ||
|
|
D-Glucose | 5.0 | g | ||
|
|
Distilled water | 960.0 | ml | ||
| 1 Dissolve ingredients (except cysteine, carbonate and glucose) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add cysteine, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 7.0, if necessary. | |||||
Salt solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
CaCl2 x 2 H2O | 0.25 | g |
|
|
MgSO4 x 7 H2O | 0.50 | g |
|
|
K2HPO4 | 1.00 | g |
|
|
KH2PO4 | 1.00 | g |
|
|
NaHCO3 | 10.00 | g |
|
|
NaCl | 2.00 | g |
|
|
Distilled water | 1000.00 | ml |