Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Replace glucose with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration.
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
|L-Cysteine HCl x H2O||0.5||g||0.5||2.847|
|1 Dissolve ingredients (except cysteine, carbonate and glucose) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add cysteine, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 7.0, if necessary.|
|Medium type:||Complex medium|
|Equipment needed:||Autoclave, Gassing station, Hungate tubes|
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
|All strains for this medium:||73|
|Strains with modifications:||15|
|Compound||Conc. [g/L]||Conc. [mM]|