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1051. TEPIDANAEROBACTER MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.14 g 0.139 1.025
2 0.20 g 0.199 0.98
3 0.15 g 0.149 1.016
4 , adjust if required 0.54 g 0.538 10.055
5 Trace element solution 1.00 ml - -
6
(0.1% w/v)
0.50 ml 5.0e-4 0.002
7 1.50 g 1.494 14.096
8 , adjust if required 2.30 g 2.291 -
9 Vitamin solution 2.00 ml - -
10 2.20 g 2.191 12.163
11 0.30 g 0.299 1.701
12 0.30 g 0.299 1.244
13 1000.00 ml 994.23 55188.1
14 Selenite-tungstate solution , might need strain adjustment 1.00 ml - -
15 , might need strain adjustment 1.10 g/l - -
Instructions:
  1. Dissolve ingredients (except carbonate, yeast extract, vitamins, glucose, cysteine and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, vitamins (sterilized by filtration), glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 7.0-7.2, if necessary.
Solution occurs in 8 other media (first defined in medium 144 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(NTA)
12.80 g 12.8 66.967
2 0.20 g 0.2 1.006
3 0.10 g 0.1 0.505
4 0.17 g 0.17 0.714
5 0.10 g 0.1 0.68
6 0.10 g 0.1 0.734
7 0.02 g 0.02 0.149
8 0.01 g 0.01 0.162
9 0.01 g 0.01 0.041
10 0.03 g 0.03 0.126
11 1.00 g 1 17.111
12 0.03 g 0.03 0.114
13 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.1
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 4
Strains with modifications: 4

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.213 55353.7
Yeast extract 2.291 -
D-Glucose 2.191 12.163
Na2CO3 1.494 14.096
NH4Cl 0.538 10.055
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Cultivation metadata from 4 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 4 strains
oxygen anaerobe 4 strains
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