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DSMZ

DSMZ 1053a . THIOPROFUNDUM LITHOTROPHICUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1053a #

ml
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.782 338.508
K2HPO4 0.09 g 0.089 0.511
KH2PO4 0.07 g 0.069 0.509
CaCl2 0.80 g 0.791 7.13
NH4Cl 0.25 g 0.247 4.623
NaNO3 1.00 g 0.989 11.637
MgSO4 x 7 H2O 4.00 g 3.956 16.052
MgCl2 x 6 H2O 3.00 g 2.967 14.596
KCl 0.33 g 0.326 4.378
Fe2(SO4)3 x H2O 0.01 g 0.01 -
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
Na2S2O3 x 5 H2O 3.00 g 2.967 11.956
NaHCO3 1.00 g 0.989 11.774
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except thiosulfate, bicarbonate and vitamins), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 6.8 with NaOH, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials up to a volume of 20% and autoclave. Add thiosulfate, vitamins and bicarbonate to the autoclaved medium from sterile anoxic stock solutions. The stock solutions of thiosulfate and vitamins are sterilized by filtration and stored under 100% N2 gas, whereas the stock solution of bicarbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust pH of the complete medium to 6.5.
2 After inoculation add 1% (v/v) sterile O2 gas (e.g., by adding 1.50 ml sterile air to 5 ml medium in a Balch-type tube) and pressurize vials to 2 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 17.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 79.7 % N2
  • 19.9 % CO2
  • 0.3 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.967 14.596
Na2S2O3 x 5 H2O 2.967 11.956
NaHCO3 0.989 11.774
NaNO3 0.989 11.637
CaCl2 0.791 7.13
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen microaerophile 1 strain
temperature thermophilic 1 strain