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DSMZ

DSMZ 1058 . THIOHALOPHILUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1058 #

ml
Equipment needed: Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 120.00 g 119.76 2049.29
K2HPO4 1.50 g 1.497 8.595
NH4Cl 0.50 g 0.499 9.329
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
CaCl2 x 2 H2O 0.05 g 0.05 0.339
MgSO4 x 7 H2O 0.50 g 0.499 2.025
Na2S2O3 x 5 H2O 5.00 g 4.99 20.106
NaHCO3 5.00 g 4.99 59.4
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride, potassium hydrogenphosphate and ammonium chloride, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution in vials suitable for anaerobic cultures (e.g. Balch-type tubes) to 50% volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere and autoclave. Add trace elements, calcium chloride, magnesium sulfate, thiosulfate, bicarbonate and vitamins from sterile stock solutions and adjust pH of the medium to 7.5 - 7.8 using a sterile stock solution of sodium carbonate (5% w/v).
2 Note: Use at least 10% (v/v) as inoculum.

Trace elements solution (Pfennig & Lippert,1966) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 12.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5 - 7.8
Equipment needed: Autoclave, Gassing station
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Gas composition

  • 80 % N2
  • 10 % CO2
  • 10 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 119.76 2049.29
NaHCO3 4.99 59.4
Na2S2O3 x 5 H2O 4.99 20.106
K2HPO4 1.497 8.595
NH4Cl 0.499 9.329
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 3 strains
nutrition chemolithoautotroph 1 strain
oxygen microaerophile 3 strains
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