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1058a. THIOHALOSPIRA HALOPHILA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 233.00 g 232.535 3978.87
2 1.50 g 1.497 8.595
3 0.50 g 0.499 9.329
4 Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
5 0.05 g 0.05 0.339
6 0.40 g 0.399 1.964
7 5.00 g 4.99 20.106
8 2.50 g 2.495 29.7
9 Seven vitamins solution 1.00 ml - -
10 1000.00 ml 996.215 55298.2
Instructions:
  1. Dissolve sodium chloride, potassium hydrogenphosphate and ammonium chloride, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution under air atmosphere in Erlenmeyer flasks to 10% of volume, close with screw caps and autoclave. Add trace elements, calcium chloride, magnesium chloride, thiosulfate, bicarbonate and vitamins from sterile stock solutions and adjust pH of the medium to 7.0 - 7.2, if necessary. Incubate with shaking.
  2. Note: Use at least 10%(v/v) as inoculum.
Solution occurs in 23 other media (first defined in medium 1369 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.20 g 2.2 7.913
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.03 g 0.03 0.485
6 0.20 g 0.2 0.841
7 0.03 g 0.03 0.176
8 0.03 g 0.03 0.126
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. pH 3.0-4.0
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave

Associated strains

All strains for this medium: 2

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 232.535 3978.87
Na2S2O3 x 5 H2O 4.99 20.106
NaHCO3 2.495 29.7
K2HPO4 1.497 8.595
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Cultivation metadata from 2 strains BacDive

halophily extreme 1 strain
halophily moderate 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
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