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1058c. THIOHALORHABDUS DENITRIFICANS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 180.00 g 179.641 3073.8
2 1.50 g 1.497 8.595
3 0.50 g 0.499 9.329
4 Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
5 0.05 g 0.05 0.339
6 0.50 g 0.499 2.025
7 5.00 g 4.99 20.106
8 5.00 g 4.99 59.4
9 Seven vitamins solution 1.00 ml - -
10 1000.00 ml 996.215 55298.2
Instructions:
  1. Dissolve sodium chloride, potassium hydrogenphosphate and ammonium chloride, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution in vials suitable for anaerobic cultures (e.g. Balch-type tubes) to 30% volume under 80% N2 and 20% CO2 gas atmosphere. Close vials with butyl rubber septa and autoclave. Add trace elements, calcium chloride, magnesium sulfate, thiosulfate, bicarbonate and vitamins from sterile stock solutions and adjust pH of the medium to 7.5 - 7.8 using a sterile stock solution of sodium carbonate (5% w/v). After inoculation, add sterile air (with hypodermic needle through the rubber closure) to a concentration of ca. 4%(v/v) O2 (e.g., add 7 ml of air to a Balch-type tube with a total volume of 27 ml).
  2. Note: Use at least 10%(v/v) as inoculum
Solution occurs in 23 other media (first defined in medium 1369 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.20 g 2.2 7.913
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.03 g 0.03 0.485
6 0.20 g 0.2 0.841
7 0.03 g 0.03 0.176
8 0.03 g 0.03 0.126
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. pH 3.0-4.0
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.6
Equipment needed: Anaerobic workbench, Autoclave

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 179.641 3073.8
NaHCO3 4.99 59.4
Na2S2O3 x 5 H2O 4.99 20.106
K2HPO4 1.497 8.595
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Cultivation metadata from 1 strains BacDive

halophily extreme 1 strain
halophily moderate 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
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