MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1058e . THIOHALOBACTER THIOCYANATICUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1058e #

ml
Equipment needed: Autoclave Gassing station Serum bottle Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 60.00 g 59.88 1024.64
K2HPO4 1.50 g 1.497 8.595
NH4Cl 0.50 g 0.499 9.329
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
CaCl2 x 2 H2O 0.05 g 0.05 0.339
MgSO4 x 7 H2O 0.50 g 0.499 2.025
Na2S2O3 x 5 H2O 5.00 g 4.99 20.106
NaHCO3 5.00 g 4.99 59.4
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride, potassium hydrogenphosphate and ammonium chloride, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution under air atmosphere in serum vials to 10% of volume, seal with butyl rubber stoppers and autoclave. Add trace elements, calcium chloride, magnesium sulfate, thiosulfate, and vitamins from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2. Thiosulfate and vitamins are sterilized by filtration. Adjust pH of the medium to 7.5 using a sterile stock solution of sodium carbonate (5% w/v). Incubate with shaking.
2 Note: Use at least 10% (v/v) as inoculum.

Trace elements solution (Pfennig & Lippert,1966) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 17.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Equipment needed: Autoclave, Filter, Gassing station, Serum bottle
Edit in Medium builder

Gas composition

  • 80 % N2
  • 18 % O2
  • 2 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 59.88 1024.64
NaHCO3 4.99 59.4
Na2S2O3 x 5 H2O 4.99 20.106
K2HPO4 1.497 8.595
NH4Cl 0.499 9.329
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain