DSMZ 1061 . MODIFIED TH AGAR

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Tryptone 5.00 g 5 70.343
Yeast extract 3.00 g 3 -
NaCl 5.00 g 5 85.558
MnSO4 x H2O 0.01 g 0.01 0.059
Trace element solution SL-6 1.00 ml - -
Agar 15.00 ml - -
Distilled water 1000.00 ml - -
1 adjust to pH 7.5
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 23.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 5 85.558
Tryptone 5 70.343
Yeast extract 3 -
MnSO4 x H2O 0.01 0.059
H3BO3 3.0e-4 0.005
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
phyla 1 2 strains
oxygen facultative aerobe 1 strain
temperature thermophilic 2 strains