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1073. AML60 MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution 1 505 ml - -
2 Solution 2 500 ml - -
Instructions:
  1. Dissolve ingredients (except carbonates, vitamins, arsenate and sulfide), boil medium for 1 min., then cool to room temperature under N2 gas atmosphere. Add solid carbonates and adjust pH to 9.8, then dispense under same gas atmosphere in culture vessels and autoclave.
  2. Prior to inoculation, add vitamins, arsenate and sulfide from sterile, anaerobic stock solutions prepared under N2 and check pH of the completed medium. The vitamin solution should be sterilized by filtration.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.198 1.499
2 25.00 mg 0.05 0.411
3 60.00 g 118.812 2032.97
4 0.15 g 0.297 1.705
5 0.08 g 0.158 1.164
6 Trace element solution SL-10 5.00 ml - -
7 Selenite-tungstate solution 0.30 ml - -
8 500.00 ml 988.324 54860.2
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.6 g 21.2 200.023
2 4.2 g 8.4 99.992
3 500.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Medium type: Defined medium
Final pH: 9.8
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1003.45 55699.7
NaCl 60 1026.65
Na2CO3 10.6 100.011
NaHCO3 4.2 49.996
K2HPO4 0.15 0.861
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