DSMZ 1073 . AML60 MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 500 ml - -
Solution 2 500 ml - -
1 Dissolve ingredients (except carbonates, vitamins, arsenate and sulfide), boil medium for 1 min., then cool to room temperature under N2 gas atmosphere. Add solid carbonates and adjust pH to 9.8, then dispense under same gas atmosphere in culture vessels and autoclave.
2 Prior to inoculation, add vitamins, arsenate and sulfide from sterile, anaerobic stock solutions prepared under N2 and check pH of the completed medium. The vitamin solution should be sterilized by filtration.
3 Growth of strain DSM 17814 takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy.
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.10 g 0.198 1.499
MgSO4 25.00 mg 0.05 0.411
NaCl 60.00 g 118.812 2033.06
K2HPO4 0.15 g 0.297 1.705
KH2PO4 0.08 g 0.158 1.164
Trace element solution SL-10 5.00 ml - -
Selenite-tungstate solution 0.30 ml - -
Distilled water 500.00 ml - -
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 10.6 g 21.2 200.023
NaHCO3 4.2 g 8.4 99.992
Distilled water 500.0 ml - -
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 176 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Defined medium
Final pH: 9.8
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 59.406 1016.53
Na2CO3 10.6 100.011
NaHCO3 4.2 49.996
K2HPO4 0.149 0.853
(NH4)2SO4 0.099 0.749
Show more