1073 .
AML60 MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
9.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution 1 | 500 | ml | ||
|
|
Solution 2 | 500 | ml | ||
| 1 Dissolve ingredients (except carbonates, vitamins, arsenate and sulfide), boil medium for 1 min., then cool to room temperature under N2 gas atmosphere. Add solid carbonates and adjust pH to 9.8, then dispense under same gas atmosphere in culture vessels and autoclave. | |||||
| 2 Prior to inoculation, add vitamins, arsenate and sulfide from sterile, anaerobic stock solutions prepared under N2 and check pH of the completed medium. The vitamin solution should be sterilized by filtration. | |||||
| 3 Growth of strain DSM 17814 takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. | |||||
Solution 1 #
505
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
(NH4)2SO4 | 0.10 | g |
|
|
MgSO4 | 25.00 | mg |
|
|
NaCl | 60.00 | g |
|
|
K2HPO4 | 0.15 | g |
|
|
KH2PO4 | 0.08 | g |
|
|
Trace element solution SL-10 | 5.00 | ml |
|
|
Selenite-tungstate solution | 0.30 | ml |
|
|
Distilled water | 500.00 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.5 | g |
|
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
|
Na2WO4 x 2 H2O | 4.0 | mg |
|
|
Distilled water | 1000.0 | ml |