DSMZ 1074 . DESULFOGLAEBA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA)
25.00 g 24.704 -
Na2SO4 3.50 g 3.459 24.349
NH4Cl 0.25 g 0.247 4.618
KH2PO4 0.20 g 0.198 1.452
Wolfe's mineral elixir 1.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
Na2CO3 1.50 g 1.482 13.985
Yeast extract 0.05 g 0.049 -
Na-pyruvate 1.00 g 0.988 8.98
Wolin's vitamin solution 10.00 ml - -
n-Decane 1.00 ml - -
Na2S x 9 H2O 0.10 g 0.099 0.411
L-Cysteine HCl x H2O 0.10 g 0.099 0.563
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, yeast extract, pyruvate, vitamins, n-decane and reducing agents) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic serum vials (e.g., Balch-type tubes or serum bottles) and autoclave. Add yeast extract, pyruvate, vitamins, n-decane and reducing agents from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Solutions of vitamins and pyruvate should be sterilized by filtration. Adjust pH of complete medium to 7.2 - 7.4.
2 Note: Use 20% (v/v) as inoculum and incubate vials in an inverted position.
Solution occurs in 23 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 12.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.4
Equipment needed: Autoclave, Filter, Gassing station
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sea Salt 24.704 -
Na2SO4 3.459 24.349
Na2CO3 1.482 13.985
Na-pyruvate 0.988 8.98
NH4Cl 0.247 4.618
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain