DSMZ 1075 . PPES-II AGAR MEDIUM

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Equipment needed:

Final pH: 7.8

Select a strain (optional):

ml
Compound Amount Unit
Peptone 2.0 g
Proteose peptone no. 3
(Difco)
1.0 g
Soytone 1.0 g
Yeast extract 1.0 g
Fe(III)-EDTA 0.1 g
Agar 15.0 g
Artificial sea water 1000.0 ml
1 Adjust pH to 7.8.

Artificial sea water #

1000
Compound Amount Unit
NaCl 30.0 g
KCl 0.7 g
MgCl2 x 6 H2O 10.8 g
MgSO4 x 7 H2O 5.4 g
CaCl2 x 2 H2O 1.0 g
Distilled water 1000.0 ml