DSMZ 1076 . SP4 MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Solution occurs in 1 other media (first defined in Medium 1076).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Tryptone 5.00 g 9.709 136.589
Peptone 2.65 g 5.146 -
PPLO broth 1.75 g 3.398 -
Distilled water 375.00 ml - -
1 Adjust pH to 7.4; autoclave at 121°C for 15 minutes.
2 Add aseptically:
Fetal bovine serum
(heat-inactivated)
90.00 ml - -
CMRL 1066
(10x concentrated; GIBCO)
25.00 ml - -
Yeast extract
(20%, autoclaved)
17.50 ml 3.5 -
Yeast extract
(20%, autoclaved)
5.00 ml 1 -
Phenol red
(1%, filter-sterilized)
1.00 ml 0.01 0.028
L-Glutamine
(200 mM; GIBCO)
1.70 ml 0.05 0.34

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 9.709 136.589
Peptone 5.146 -
PPLO broth 3.398 -
Yeast extract 1 -
L-Glutamine 0.05 0.34
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Cultivation metadata from 1 strains BacDive

phyla 2 2 strains
oxygen microaerophile 1 strain
temperature mesophilic 1 strain