1076a
1076a .
SP4 MEDIUM WITH GLUCOSE
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1076a #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 1076 | 517.0 | ml | - | - | |
|
Glucose
(10 %) |
5.2 | ml | 0.52 | 2.886 | |
| 1 Use medium 1076, but add 5.2 ml 10% glucose (filter-sterilized). | |||||
Solution occurs in 1
other media (first defined in Medium 1076).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 5.00 | g | 9.709 | 136.589 | |
| Peptone | 2.65 | g | 5.146 | - | |
| PPLO broth | 1.75 | g | 3.398 | - | |
| Distilled water | 375.00 | ml | - | - | |
| 1 Adjust pH to 7.4; autoclave at 121°C for 15 minutes. | |||||
| 2 Add aseptically: | |||||
|
Fetal bovine serum
(heat-inactivated) |
90.00 | ml | - | - | |
|
CMRL 1066
(10x concentrated; GIBCO) |
25.00 | ml | - | - | |
|
Yeast extract
(20%, autoclaved) |
17.50 | ml | 3.5 | - | |
|
Yeast extract
(20%, autoclaved) |
5.00 | ml | 1 | - | |
|
Phenol red
(1%, filter-sterilized) |
1.00 | ml | 0.01 | 0.028 | |
|
L-Glutamine
(200 mM; GIBCO) |
1.70 | ml | 0.05 | 0.34 | |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.4 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Tryptone | 9.653 | 135.801 |
| Peptone | 5.116 | - |
| PPLO broth | 3.378 | - |
| Yeast extract | 0.994 | - |
| Glucose | 0.52 | 2.886 |
| Show more | ||
