DSMZ 1076b . SP4-Z MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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* Modified for Mycoplasmoides pneumoniae DSM 23978

Plus glucose; aerobic plus 5% CO2

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
1 The difference between this medium and "normal" SP4 medium (Medium 1076) is that the yeast extract was left out and DNA was added instead. Since most mycoplasmas use either glucose or arginine as their energy source one is added to the media. Do not combine both in one medium, arginine can inhibit the growth of some glucose utilizers (unknown reason).
Compound Amount Unit Conc. [g/L] Conc. [mM]
PPLO broth 0.70 g 3.5 -
Tryptone 2.00 g 10 140.687
Bacto peptone
(BD)
1.00 g 5 -
Distilled water 130.00 ml - -
DNA
(fish sperm; SERVA)
0.04 g 0.2 -
2 Adjust pH to 7.4; autoclave at 121°C for 15 minutes.
3 Add aseptically:
CMRL 1066
(10x concentrated; GIBCO)
10.00 ml - -
Yeast extract
(BD; 2%, autoclaved)
20.00 ml 0.4 -
Fetal bovine serum
(heat-inactivated)
17.00 ml - -
Swine serum
(heat-inactivated)
17.00 ml - -
4 As energy source add either:
Glucose OR L-Arginine x HCl
(50 % aqueous)
2.00 ml 1 5.551
5 For Ureaplasma add instead of Glucose or Arginine:
Urea 0.40 g 2 33.302
Agar , for solid medium 2.00 g 10 29.733
6 For agar plates use only 126 ml of distilled water and add 2 g of agar. Medium containing agar should be placed at 55°C before adding supplements.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
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Gas composition

  • 5 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 109
Strains with modifications: 109
Mycoplasmoides pneumoniae DSM 23978

  5% CO2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tryptone 10 140.687
Agar 10 29.733
Bacto peptone 5 -
PPLO broth 3.5 -
Urea 2 33.302
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Cultivation metadata from 109 strains BacDive

phyla 3 100 strains
oxygen microaerophile 29 strains
oxygen aerobe 8 strains
oxygen facultative anaerobe 3 strains
oxygen facultative aerobe 1 strain
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