1077 .
PH MEDIUM
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
Solution 1 #
80
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
PPLO broth | 1.68 | g | ||
|
|
Distilled water | 80.00 | ml | ||
| 1 Adjust pH to 6.5 and autoclave. | |||||
Solution 2 #
20
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Horse serum
(inactivated) |
18.700 | ml | ||
|
|
Yeast extract
(25%, autoclaved) |
0.936 | ml | ||
|
|
Fish-Sperm DNA
(filter-sterilized) |
0.187 | ml | ||
|
|
Distilled water | 0.187 | ml | ||
| 1 Adjust pH to 7.8. | |||||