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1078. FRIIS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.820 g 6.406 -
2
(GIBCO)
0.870 g 6.797 -
3 Hank's BSS 50.000 ml - -
4 78.000 ml 608.282 33764.8
5
(heat-inactivated)
30.000 ml - -
6
(25% , autoclaved)
3.320 ml - -
7
(1% , filter-sterilized)
0.133 ml - -
8
(autoclaved)
0.343 ml 2.675 148.478
Instructions:
  1. Adjust to pH 7.4.
  2. After autoclaving add: #5-8
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 4.0000 g 8 136.887
3 0.2000 g 0.4 5.365
4 0.0500 g 0.1 0.406
5 0.0500 g 0.1 0.492
6 50.0000 ml 99.821 5540.88
7 0.0925 g 0.185 1.667
8 0.0750 g 0.15 0.843
9 0.0300 g 0.06 0.441
10 450.0000 ml 898.386 49868
Instructions:
  1. 25 ml solution 1 ( 4 g NaCl, 0.2 g KCl, 0.05 g MgSO4 x 7 H2O, 0.05 g MgCl2 x 6 H2O dissolved in 25 ml distilled water; then 0.0925 g CaCl2 added and dissolved) and 25 ml of solution 2 ( 0.075 g Na2HPO4 x 2 H2O, 0.03 g KH2PO4 dissolved in 25 ml distilled water) are autoclaved separately and then combined with 450 ml sterile H2O.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave

Associated strains

All strains for this medium: 3
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1000.88 55557.4
PPLO broth 6.797 -
Bacto brain heart infusion 6.406 -
NaCl 3.125 53.472
KCl 0.156 2.096
CaCl2 0.072 0.651
Na2HPO4 x 2 H2O 0.059 0.329
MgCl2 x 6 H2O 0.039 0.192
MgSO4 x 7 H2O 0.039 0.158
KH2PO4 0.023 0.172
Porcine serum - -
Yeast extract - -
Phenol red solution - -

Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen aerobe 2 strains
temperature mesophilic 3 strains

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