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DSMZ

DSMZ 1078 . FRIIS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1078 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto brain heart infusion 0.820 g 5.093 -
PPLO broth
(GIBCO) 		0.870 g 5.404 -
Hank's BSS
(BSS) 		50.000 ml - -
Distilled water 78.000 ml - -
1 Adjust to pH 7.4.
2 After autoclaving add:
Porcine serum
(heat-inactivated) 		30.000 ml - -
Yeast extract
(autoclaved, 25%) 		3.320 ml 0.83 -
Phenol red
(filter-sterilized, 1%) 		0.133 ml 0.001 0.004
Distilled water
(autoclaved) 		0.343 ml - -

Hank's BSS #

500
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 25 ml - -
Solution 2 25 ml - -
Water 450 ml - -
1 25 ml solution 1 (4 g NaCl, 0.2 g KCl, 0.05 g MgSO4 x 7 H2O, 0.05 g MgCl2 x 6 H2O dissolved in 25 ml distilled water; then 0.0925 g CaCl2 added and dissolved) and 25 ml of solution 2 (0.075 g Na2HPO4 x 2 H2O, 0.03 g KH2PO4 dissolved in 25 ml distilled water) are autoclaved separately and then combined with 450 ml sterile H2O.

Solution 1 #

25
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 4.0000 g 160 2737.85
KCl 0.2000 g 8 107.311
MgSO4 x 7 H2O 0.0500 g 2 8.114
MgCl2 x 6 H2O 0.0500 g 2 9.838
Distilled water 25.0000 ml - -
CaCl2 0.0925 g 3.7 33.339

Solution 2 #

25
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 0.075 g 3 21.133
KH2PO4 0.030 g 1.2 8.818
Distilled water 25.000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 4
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
PPLO broth 5.404 -
Bacto brain heart infusion 5.093 -
NaCl 2.484 42.513
Yeast extract 0.83 -
KCl 0.124 1.666
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Cultivation metadata from 4 strains BacDive

phyla 1 3 strains
oxygen aerobe 2 strains
temperature mesophilic 3 strains