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1079. MYCOPLASMA/ACHOLEPLASMA MEDIUM (CIP MEDIUM 88)

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 17 g 17 -
2
(25% , pH 7.0, autoclaved)
100 ml - -
3
(1% , pH 7.0, autoclaved)
20 ml - -
4 670 ml 668.799 37123.9
5 Sterile supplement 210 ml - -
Instructions:
  1. Add all components except sterile supplement; adjust pH to 7.6 + 0.2. Autoclave at 121°C for 15 minutes; add aseptically sterile supplement.
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(50 %)
10 ml - -
2 200 ml - -
3 1 g 4.762 13.629
Instructions:
  1. Adjust pH to 7.0 + 0.2 and autoclave at 121°C for 15 minutes.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 668.799 37123.9
PPLO broth 17 -
Ampicillin 1 2.862
Horse serum - -
Glucose - -
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