1079
1079 .
MYCOPLASMA/ACHOLEPLASMA MEDIUM (CIP MEDIUM 88)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1079 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| PPLO broth | 17 | g | 17 | - | |
|
Yeast extract
(pH 7.0, autoclaved, 25%) |
100 | ml | 25 | - | |
|
Phenol red
(pH 7.0, autoclaved, 1%) |
20 | ml | 0.2 | 0.564 | |
| Distilled water | 670 | ml | - | - | |
| Sterile supplement | 210 | ml | - | - | |
| 1 Add all components except sterile supplement; adjust pH to 7.6 + 0.2. Autoclave at 121°C for 15 minutes; add aseptically sterile supplement. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Glucose
(50 %) |
10 | ml | 5 | 27.753 | |
| Horse serum | 200 | ml | - | - | |
| Ampicillin | 1 | g | 4.762 | 13.629 | |
| 1 Adjust pH to 7.0 + 0.2 and autoclave at 121°C for 15 minutes. | |||||
Metadata
| Last modified: | 26.03.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 7.4 - 7.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |