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DSMZ

DSMZ 1080 . MYCOPLASMA MEDIUM (CIP MEDIUM 89)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1080 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
PPLO broth 17 g 17 -
Yeast extract
(pH 7.0, autoclaved, 25%) 		100 ml 25 -
Phenol red
(pH 7.0, autoclaved, 1%) 		20 ml 0.2 0.564
Distilled water 670 ml - -
Sterile supplement 210 ml - -
1 Add all components except sterile supplement; adjust pH to 7.2 + 0.2. Autoclave at 121°C for 15 minutes; add aseptically sterile supplement.

Sterile supplement #

210
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Arginine solution 50%(w/v) 10 ml - -
Horse serum 200 ml - -
Ampicillin 1 g 4.762 13.629
1 Autoclave at 121°C for 15 minutes.

Arginine solution 50%(w/v) #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Arginine x HCl 50 g 500 -
Distilled water 100 ml - -
1 50 g L-Arginine hydrochloride plus distilled water to make up to 100 ml.

Metadata

Last modified: 26.03.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.0 - 7.4
Equipment needed: Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 25 -
PPLO broth 17 -
L-Arginine x HCl 5 -
Ampicillin 1 2.862
Phenol red 0.2 0.564
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