DSMZ 1082 . AMINIPHILUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.297 2.183
K2HPO4 0.3 g 0.297 1.705
NH4Cl 1.0 g 0.99 18.51
NaCl 10.0 g 9.901 169.421
KCl 0.1 g 0.099 1.328
CaCl2 x 2 H2O 0.1 g 0.099 0.673
MgCl2 x 6 H2O 0.5 g 0.495 2.435
Modified Wolin's mineral solution 10.0 ml - -
Trypticase peptone
(BD BBL)
10.0 g 9.901 -
Yeast extract 2.0 g 1.98 -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
L-Cysteine HCl x H2O 0.5 g 0.495 2.819
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except cysteine), then sparge medium for 30 - 45 min with 100% N2 gas atmosphere to make it anoxic. Add cysteine, adjust pH to 7.1 and dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. The pH of the complete medium should be 7.0 - 7.2.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase peptone 9.901 -
Yeast extract 1.98 -
NH4Cl 0.99 18.51
L-Cysteine HCl x H2O 0.495 2.819
MgCl2 x 6 H2O 0.495 2.435
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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