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1086. DESULFATIRHABDIUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.53 g 0.514 2.885
2 0.41 g 0.397 2.919
3 0.30 g 0.291 5.435
4 0.11 g 0.107 0.725
5 0.10 g 0.097 0.477
6 0.30 g 0.291 4.974
7 2.80 g 2.713 19.101
8 Trace element solution SL-10 1.00 ml - -
9 Selenite-tungstate solution 1.00 ml - -
10 0.02 g 0.019 -
11
(0.1% w/v)
0.50 ml 4.8e-4 0.002
12 1.50 g 1.453 13.714
13 Vitamin solution 10.00 ml - -
14 Na-crotonate solution (1 M) 20.00 ml - -
15 0.43 g 0.417 2.891
16 0.50 g 0.484 2.017
17 1000.00 ml 967.255 53690.7
Instructions:
  1. Dissolve ingredients (except carbonate, vitamins, crotonate, benzoate and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, crotonate, benzoate and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The crotonate and vitamin solutions should be sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2. After inoculation pressurize the vessels with sterile 80% N2 and 20% CO2 gas mixture to 0.7 bar overpressure.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
Solution occurs in 3 other media (first defined in medium 870 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(ALDRICH 113018)
0.86 g 86 998.964
2
(10 N )
1.00 ml 213 5325.39
3 9.00 ml 898.386 49868
Instructions:
  1. Dissolve crotonic acid in 8 ml distilled water, add around 1 ml of 10 N NaOH and adjust pH to around 7. Then add water to reach a volume of 10 ml. Sterilize by filtration under 100% N2 gas atmosphere.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.1
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.263 55300.9
NaOH 4.128 103.217
Na2SO4 2.713 19.101
Crotonic acid 1.667 19.36
Na2CO3 1.453 13.714
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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