108 .
ACIDIPHILIUM ACIDOPHILUM MEDIUM
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Equipment needed:
Autoclave
Final pH:
4.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 3.00 | g | ||
|
|
KH2PO4 | 0.50 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
KCl | 0.10 | g | ||
|
|
Ca(NO3)2 x 4 H2O | 18.00 | mg | ||
|
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
0.01 | ml | ||
|
|
Agar
, for solid medium
optional |
15.00 | g | ||
|
|
D-Glucose | 10.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 In liquid medium, the pH should be adjusted to 3.5 with H2SO4. In solid medium, the pH should be adjusted to 4.5 with H2SO4 after autoclaving the medium. Sterilize the glucose separately and add to the sterile medium. | |||||