1091 .
HALOQUADRATUM WALSBYI MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 195.00 | g | ||
|
|
MgSO4 x 7 H2O | 50.00 | g | ||
|
|
MgCl2 x 6 H2O | 35.00 | g | ||
|
|
KCl | 5.00 | g | ||
|
|
NaHCO3 | 0.25 | g | ||
|
|
NaNO3 | 1.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.50 | g | ||
|
|
KH2PO4 | 0.05 | g | ||
|
|
NH4Cl | 0.03 | g | ||
|
|
Tris buffer
(1M) |
20.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Add after autoclaving: | |||||
|
|
Yeast extract | 0.05 | g | ||
|
|
Sodium pyruvate | 1.00 | g | ||
| 2 Final pH 7.4 | |||||
| 3 Yeast extract and sodium pyruvate are added from sterile (autoclaved) stock solutions to the respective concentrations. | |||||