Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Sea Salt
(SIGMA) |
30.0 | g | 29.91 | - | |
Wolfe's mineral elixir | 1.0 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
Sulfur
(powdered) |
3.0 | g | 2.991 | 93.266 | |
NaHCO3 | 2.5 | g | 2.493 | 29.67 | |
Yeast extract | 2.0 | g | 1.994 | - | |
Wolin's vitamin solution (10x) | 2.0 | ml | - | - | |
Na2S x 9 H2O | 0.5 | g | 0.499 | 2.076 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve ingredients (except sulfur, bicarbonate, yeast extract, vitamins and sulfide) and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic serum vials (e.g., Balch-type tubes) containing already the appropriate amount of sulfur, only to 30% of their volume to allow for a large headspace. Autoclave at 110°C for 20 min. Add yeast extract, vitamins and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust pH of the complete medium to 7.0-7.2. | |||||
2 After inoculation pressurize vessels with sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgSO4 x 7 H2O | 30.00 | g | 30 | 121.716 | |
MnSO4 x H2O | 5.00 | g | 5 | 29.583 | |
NaCl | 10.00 | g | 10 | 171.116 | |
FeSO4 x 7 H2O | 1.00 | g | 1 | 3.597 | |
CoCl2 x 6 H2O | 1.80 | g | 1.8 | 13.863 | |
CaCl2 x 2 H2O | 1.00 | g | 1 | 6.802 | |
ZnSO4 x 7 H2O | 1.80 | g | 1.8 | 6.26 | |
CuSO4 x 5 H2O | 0.10 | g | 0.1 | 0.401 | |
AlK(SO4)2 x 12 H2O | 0.18 | g | 0.18 | 0.379 | |
H3BO3 | 0.10 | g | 0.1 | 1.617 | |
Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
(NH4)2Ni(SO4)2 x 6 H2O | 2.80 | g | 2.8 | 7.089 | |
Na2WO4 x 2 H2O | 0.10 | g | 0.1 | 0.303 | |
Na2SeO4 | 0.10 | g | 0.1 | 0.529 | |
Distilled water | 1000.00 | ml | - | - | |
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 27.10.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 - 7.2 |
Equipment needed: | Autoclave, Filter, Gassing station |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Sea Salt | 29.91 | - |
Sulfur | 2.991 | 93.266 |
NaHCO3 | 2.493 | 29.67 |
Yeast extract | 1.994 | - |
Na2S x 9 H2O | 0.499 | 2.076 |
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