DSMZ 1095 . NATRANAEROBIUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 100.0 g 98.912 1692.54
KH2PO4 0.2 g 0.198 1.454
MgCl2 0.1 g 0.099 1.039
NH4Cl 0.5 g 0.495 9.246
Yeast extract
(OXOID)
10.0 g 9.891 -
Tryptone
(BD Bacto)
10.0 g 9.891 -
Trace element solution SL-10 1.0 ml - -
Na2CO3 68.0 g 67.26 634.601
NaHCO3 38.0 g 37.587 447.422
L-Cysteine HCl x H2O 0.7 g 0.692 3.942
Wolin's vitamin solution 10.0 ml - -
Sucrose 5.0 g 4.946 14.448
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except carbonates, cysteine, vitamins and sucrose), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonate, hydrogencarbonate and cysteine and adjust pH to 8.5. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add vitamins and sucrose from sterile anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 12.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 98.912 1692.54
Na2CO3 67.26 634.601
NaHCO3 37.587 447.422
Tryptone 9.891 -
Yeast extract 9.891 -
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Cultivation metadata from 4 strains BacDive

pH 8 - 10 2 strains
pH 10 - 12 2 strains
phyla 1 4 strains
oxygen anaerobe 3 strains
temperature thermophilic 4 strains