1095
1095 .
NATRANAEROBIUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Natranaerobius sp. DSM 23380
* Modification: Adjust pH of medium to 9.5 and replace sucrose with 5.0 g/l D-glucose added from a sterile anoxic stock solution after autoclaving.
Growth conditions: anaerobic
45 °C
Main sol. 1095 #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 100.0 | g | 99.8 | 1707.74 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.467 | |
| MgCl2 | 0.1 | g | 0.1 | 1.048 | |
| NH4Cl | 0.5 | g | 0.499 | 9.329 | |
|
Yeast extract
(OXOID) |
10.0 | g | 9.98 | - | |
|
Tryptone
(BD Bacto) |
10.0 | g | 9.98 | - | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Na2CO3 | 68.0 | g | 67.864 | 640.301 | |
| NaHCO3 | 38.0 | g | 37.924 | 451.44 | |
| L-Cysteine HCl x H2O | 0.7 | g | 0.699 | 3.978 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Sucrose* | 5.0 | g | 4.99 | 14.578 | |
| D-Glucose* | 5.0 | g | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonates, cysteine, sucrose and vitamins), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonate, hydrogencarbonate and cysteine and adjust pH to 8.5. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sucrose and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Solution occurs in 306
other media (first defined in Medium 120).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 20 | mg | 0.02 | 0.082 |
| Folic acid | 20 | mg | 0.02 | 0.045 |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 |
| Riboflavin | 50 | mg | 0.05 | 0.133 |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 |
| Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
| (DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 16.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | * 9.5 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 99.8 | 1707.74 |
| Na2CO3 | 67.864 | 640.301 |
| NaHCO3 | 37.924 | 451.44 |
| Tryptone | 9.98 | - |
| Yeast extract | 9.98 | - |
| Show more | ||
