This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 700 | ml | - | - | |
Solution B | 305 | ml | - | - | |
1 Adjust solution A for final pH 6.0 with HCl. Autoclave solution A at 121°C for 15 min. Cool to room temperature and aseptically add solution B filter sterilised. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
PPLO broth | 14.70 | g | 21 | - |
L-Cysteine HCl | 0.10 | g | 0.143 | 0.906 |
Fish-Sperm DNA | 0.20 | g | 0.286 | - |
Phenol red | 0.02 | g | 0.029 | 0.081 |
Urea | 0.40 | g | 0.571 | 9.515 |
Water | 700.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Yeast extract
(BD, 2%) |
100 | ml | 2 | - |
Horse serum | 200 | ml | - | - |
Isovitalex | 5 | ml | - | - |
Last modified: | 24.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.0 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
PPLO broth | 14.627 | - |
Yeast extract | 0.607 | - |
Urea | 0.398 | 6.627 |
Fish-Sperm DNA | 0.199 | - |
L-Cysteine HCl | 0.1 | 0.631 |
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