1096 .
UREAPLASMA MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
6.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 700 | ml | ||
|
|
Solution B | 305 | ml | ||
| 1 Adjust solution A for final pH 6.0 with HCl. Autoclave solution A at 121°C for 15 min. Cool to room temperature and aseptically add solution B filter sterilised. | |||||
Solution A #
700
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
PPLO broth | 14.70 | g |
|
|
L-Cysteine HCl | 0.10 | g |
|
|
Fish-Sperm DNA | 0.20 | g |
|
|
Phenol red | 0.02 | g |
|
|
Urea | 0.40 | g |
|
|
Water | 700.00 | ml |
Solution B #
305
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Yeast extract
(BD, 2%) |
100 | ml |
|
|
Horse serum | 200 | ml |
|
|
Isovitalex | 5 | ml |