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DSMZ

DSMZ 1098 . ARCHAEOGLOBUS INFECTUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1098 #

ml
Equipment needed: Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA) 		35.00 g 34.93 -
NH4Cl 0.10 g 0.1 1.866
KH2PO4 0.05 g 0.05 0.367
Na-acetate 1.60 g 1.597 19.465
Yeast extract 0.10 g 0.1 -
Wolfe's mineral elixir 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
NaHCO3 2.00 g 1.996 23.76
2-Mercaptoethanesulfonic acid
(coenzyme M) 		0.14 g 0.14 0.983
Seven vitamins solution 1.00 ml - -
Na2S2O3 x 5 H2O 2.50 g 2.495 10.053
DL-Dithiothreitol 0.30 g 0.299 1.94
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, coenzyme M, vitamins, thiosulfate, and dithiothreitol), then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic serum vials (e.g., Balch-type tubes) to 30% of their volume and autoclave. Add coenzyme M, vitamins, thiosulfate, and dithiothreitol from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The vitamin and dithiothreitol solutions should be sterilized by filtration. Adjust the pH of the complete medium to 6.5.
2 After inoculation pressurize culture vessels to 1.5 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.

Wolfe's mineral elixir #

1000
Solution occurs in 21 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 09.06.23
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sea Salt 34.93 -
Na2S2O3 x 5 H2O 2.495 10.053
NaHCO3 1.996 23.76
Na-acetate 1.597 19.465
DL-Dithiothreitol 0.299 1.94
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain