Anaerobic. Rehydrate and grow lyophilized cells from the ampoule in liquid broth 10. Subsequently, subculturing may be carried out in liquid medium or on agar plates.
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Bacto peptone | 10 | g | ||
|
Yeast extract | 10 | g | ||
|
Glucose | 20 | g | ||
|
Agar , for solid medium | 15 | g | ||
|
Distilled water | 1000 | ml | ||
1 Incubate microaerobic, for example in a candle jar. |