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110. CHOPPED MEAT MEDIUM WITH CARBOHYDRATES

Culture medium recipe

Vol.
×

M 110 #

ml
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(fat free)
500.0 g 478.011 -
2 1000.0 ml 954.309 52972.1
3
(1 N)
25.0 ml 50.908 1272.8
4 30.0 g 28.681 -
5 5.0 g 4.78 -
6 5.0 g 4.78 27.444
7
(0.1% w/v)
0.5 ml 4.8e-4 0.002
8 4.0 g 3.824 21.227
9 1.0 g 0.956 2.793
10 1.0 g 0.956 2.793
11
(soluble)
1.0 g 0.956 -
16
optional
0.5 g/l 0.5 3.172
17
optional
15.0 g 14.34 -
Instructions:
  1. Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: #4-11
  2. To make medium anoxic boil it, cool under 100% N2 gas atmosphere, add 0.5 g/l L-cysteine hydrochloride and adjust pH to 7.0. Dispense under same gas atmosphere 7 ml medium into Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121°C for 30 min. For agar slants use 15.0 g agar per 1000.0 ml medium.
  3. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000.0 ml of medium after autoclaving: #12 and either #13 or #14
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 50 mg 0.495 -
2
(1 N)
1 ml 21.089 527.266
3 100 ml 988.324 54860.2
Instructions:
  1. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

SL 312: Vitamin K1 solution #

20
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.1 ml - -
2
(95 %)
20.0 ml 750.5 16291
Instructions:
  1. Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

SL 342: Vitamin K3 solution #

100
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 5 g/l 5 -
3
(95 %)
x - -
4 100 ml 998.207 55408.8
Instructions:
  1. Dissolve 5 mg/ml of vitamin K3 in 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 224
Strains with modifications: 219
110 CHOPPED MEAT MEDIUM WITH CARBOHYDRATES
a CMC MEDIUM (N2/CO2)

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 954.309 52972.1
Ground beef 478.011 -
NaOH 50.908 1272.8
Casitone 28.681 -
Agar
optional
14.34 -
K2HPO4 4.78 27.444
Yeast extract 4.78 -
D-Glucose 3.824 21.227
Cellobiose 0.956 2.793
Maltose 0.956 2.793
Starch 0.956 -
L-Cystein HCl
optional
0.5 3.172
Na-resazurin 4.8e-4 0.002

Cultivation metadata from 224 strains BacDive

halophily not 6 strains
halophily slightly 1 strain
halophily moderate 1 strain
pH 6 - 8 15 strains
pH 8 - 10 12 strains
pH 4 - 6 11 strains
pH 2 - 4 2 strains
phyla 7 215 strains
oxygen anaerobe 186 strains
oxygen obligate anaerobe 9 strains
oxygen facultative anaerobe 2 strains
oxygen microaerophile 1 strain
oxygen aerobe 1 strain
temperature mesophilic 214 strains
temperature psychrophilic 6 strains
temperature thermophilic 4 strains

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J461. CHOPPED MEAT MEDIUM 99 %

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