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1100. DESULFACINUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 2.991 21.057
2 0.20 g 0.199 1.465
3 0.30 g 0.299 5.592
4 7.00 g 6.979 119.418
5 Trace element solution SL-11 1.00 ml - -
6 Selenite-tungstate solution 1.00 ml - -
7 3.10 g 3.091 15.203
8 0.50 g 0.499 6.687
9 0.15 ml - -
10 0.50 g 0.499 -
11
(0.1% w/v)
0.50 ml 5.0e-4 0.002
12 1.50 g 1.496 17.802
13 2.00 g 1.994 17.794
14 0.50 g 0.499 2.076
15 1000.00 ml 995.221 55243.1
Instructions:
  1. Dissolve ingredients (except bicarbonate, lactate and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add lactate and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The sulfide stock solution should be neutralized with H2SO4 prior to sterilization (see medium 29). Adjust pH of the complete medium to 7.2, if necessary.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.211 55353.6
NaCl 6.979 119.418
MgCl2 x 6 H2O 3.091 15.203
Na2SO4 2.991 21.057
Na-L-lactate 1.994 17.794
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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