Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 25.00 | g | 24.925 | 426.51 | |
K2HPO4 | 0.50 | g | 0.499 | 2.862 | |
Yeast extract | 0.20 | g | 0.199 | - | |
Na2SO4 | 3.00 | g | 2.991 | 21.058 | |
NaHCO3 | 1.85 | g | 1.844 | 21.956 | |
Na2CO3 | 1.20 | g | 1.196 | 11.288 | |
MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.49 | |
Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
Na-L-lactate | 2.20 | g | 2.193 | - | |
Seven vitamins solution | 1.00 | ml | - | - | |
Na2S x 9 H2O | 0.50 | g | 0.499 | 2.076 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except carbonates, magnesium chloride, trace elements, lactate, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then add and dissolve carbonates, adjust pH to 9.0, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add magnesium chloride, trace elements, lactate, vitamins and sulfide from sterile anoxic stock solution prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Adjust pH of complete medium to 9.0 - 9.5, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
EDTA | 5.00 | g | 5 | 17.109 | |
FeSO4 x 7 H2O | 2.20 | g | 2.2 | 7.913 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
H3BO3 | 0.03 | g | 0.03 | 0.485 | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
CuCl2 x 2 H2O | 0.03 | g | 0.03 | 0.176 | |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
Distilled water | 1000.00 | ml | - | - | |
1 pH 3.0-4.0 |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.5 | g | 0.5 | 12.501 |
Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 100 | mg | 0.1 | 0.074 |
p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 |
D-(+)-biotin | 20 | mg | 0.02 | 0.082 |
Nicotinic acid | 200 | mg | 0.2 | 1.625 |
Calcium pantothenate | 100 | mg | 0.1 | 0.21 |
Pyridoxine hydrochloride | 300 | mg | 0.3 | 1.459 |
Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | 0.536 |
Distilled water | 1000 | ml | - | - |
Last modified: | 16.09.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 9.0 - 9.5 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 24.925 | 426.51 |
Na2SO4 | 2.991 | 21.058 |
Na-L-lactate | 2.193 | - |
NaHCO3 | 1.844 | 21.956 |
Na2CO3 | 1.196 | 11.288 |
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