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DSMZ 1102 . MEDIUM FOR ENHYDROMYXA SALSUGINOSA

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(III) citrate 0.02 g 0.02 0.082
CaCl2 x 2 H2O 0.20 g 0.2 1.36
MgSO4 x 7 H2O 1.60 g 1.6 6.492
KCl 0.10 g 0.1 1.341
NaCl 10.00 g 10 171.116
Na-caseinate 0.50 g 0.5 -
HEPES 2.00 g 2 8.393
NaHCO3 0.16 g 0.16 1.905
H3BO3 20.00 mg 0.02 0.323
KBr 80.00 mg 0.08 0.672
SrCl2 x 6 H2O 30.00 mg 0.03 0.113
Agar 20.00 g 20 59.465
Trace element solution SL-4 2.00 ml - -
Distilled water 998.00 ml - -
Vitamin B12 0.50 mg 5.0e-4 3.7e-4
1 Adjust to pH 7.2, check and adjust pH after autoclaving. Add vitamin B12 from a filter sterilized stock solution after autoclaving.
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.465
NaCl 10 171.116
HEPES 2 8.393
MgSO4 x 7 H2O 1.6 6.492
Na-caseinate 0.5 -
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
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