1102 .
MEDIUM FOR ENHYDROMYXA SALSUGINOSA
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Equipment needed:
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Fe(III) citrate | 0.02 | g | ||
|
|
CaCl2 x 2 H2O | 0.20 | g | ||
|
|
MgSO4 x 7 H2O | 1.60 | g | ||
|
|
KCl | 0.10 | g | ||
|
|
NaCl | 10.00 | g | ||
|
|
Na-caseinate | 0.50 | g | ||
|
|
HEPES | 2.00 | g | ||
|
|
NaHCO3 | 0.16 | g | ||
|
|
H3BO3 | 20.00 | mg | ||
|
|
KBr | 80.00 | mg | ||
|
|
SrCl2 x 6 H2O | 30.00 | mg | ||
|
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Agar | 20.00 | g | ||
|
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Trace element solution SL-4 | 2.00 | ml | ||
|
|
Distilled water | 998.00 | ml | ||
|
|
Vitamin B12 | 0.50 | mg | ||
| 1 Adjust to pH 7.2, check and adjust pH after autoclaving. Add vitamin B12 from a filter sterilized stock solution after autoclaving. | |||||
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 0.20 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
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MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.30 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. | |||||