DSMZ 110a . CMC MEDIUM (N2/CO2)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casitone 30.0 g 30 -
Yeast extract 5.0 g 5 -
K2HPO4 5.0 g 5 28.707
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
L-Cysteine HCl x H2O 0.5 g 0.5 2.847
D-Glucose 4.0 g 4 22.203
Cellobiose 1.0 g 1 2.921
Maltose 1.0 g 1 2.921
Starch
(soluble)
1.0 g 1 -
Na2CO3 1.5 g 1.5 14.153
Meat filtrate 1000.0 ml - -
1 Dissolve ingredients, except cysteine, carbohydrates and carbonate, boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere, add 0.5 g/l L-cysteine-HCl x H2O and dispense under same gas atmosphere into Hungate-type tubes (for strains demanding meat particles put those first into the tube, use 1 part meat particles to 4 or 5 parts fluid). Autoclave at 121°C for 20 min. After autoclaving add glucose, cellobiose, maltose and starch from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of medium to pH 7.0, if necessary.
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ground beef
(fat free)
500 g 500 -
NaOH
(1 N)
25 ml - -
Distilled water 1000 ml - -
1 Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000.0 ml.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 46

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
K2HPO4 5 28.707
Yeast extract 5 -
D-Glucose 4 22.203
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Cultivation metadata from 46 strains BacDive

pH 6 - 8 2 strains
pH 4 - 6 1 strain
phyla 2 41 strains
oxygen anaerobe 12 strains
temperature mesophilic 47 strains