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1110. NAUTILIA PROFUNDICOLA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.00 g 19.763 338.159
2 3.00 g 2.964 14.581
3 0.15 g 0.148 1.008
4 0.50 g 0.494 6.627
5 0.25 g 0.247 4.618
6 0.20 g 0.198 1.452
7 Trace element solution SL-10 1.00 ml - -
8 Selenite-tungstate solution 1.00 ml - -
9
(0.1% w/v)
0.50 ml 4.9e-4 0.002
10 1.50 g 1.482 13.985
11 Vitamin solution 10.00 ml - -
12
(powdered)
5.00 g 4.941 154.079
13 0.50 g 0.494 2.057
14 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients (except sulfur, carbonate, vitamins and sulfide), then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (to 20% of volume) that contain already the appropriate amount of sulfur. For sterilization heat cultivation vessels in a water bath to 90 - 100°C for 1 - 2 hours on each of three successive days. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The vitamin solution should be sterilized by filtration. Adjust the pH of the complete medium to 7.0. After inoculation, pressurize culture vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % H2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.198 55408.3
NaCl 19.763 338.159
Sulfur 4.941 154.079
MgCl2 x 6 H2O 2.964 14.581
Na2CO3 1.482 13.985
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Cultivation metadata from 1 strains BacDive

halophily moderate 1 strain
halophily slightly 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
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